Development of Efficient in Vitro Regeneration Protocol for Dendrocalamus stocksii (Munro.) Through Nodal Explants
Priyadarshini K. C.
Development of Efficient in Vitro Regeneration Protocol for Dendrocalamus stocksii (Munro.) Through Nodal Explants - M.Sc. (Forest) - Dharwad University of Agricultural Sciences 2024 - 117 32 Cms
ABSTRACT
Bamboo, a versatile and rapidly growing woody species, holds significant importance across various aspects of life and culture. Among the economically and ecologically potential bamboo species distributed in India, Dendrocalamus stocksii plays an important role in fulfilling those demands. To address gaps, particularly concerning the development of protocols for axillary shoot proliferation using nodal explants, is crucial. Therefore, in vitro propagation study was conducted with the objectives to know the influence of plant growth regulators on in vitro shoot initiation, in vitro shoot multiplication and in vitro rooting of nodal explants.
The research evaluated various nutrient media combinations to optimize shoot initiation, multiplication and rooting processes. The early shoot initiation (3.67 days) was achieved using MS media supplemented with additives+ NAA (0.2 mg/l) + BAP (3.0 mg/l), which also resulted in a maximum of 5 shoots. The greatest shoot length was observed with MS + additives + NAA (0.2 mg/l) + BAP (1.0 mg/l). For in vitro shoot multiplication, the highest sprouting and survival percentages were obtained with MS + additives + NAA (0.2 mg/l) + BAP (4.0 mg/l). The maximum shoot number during multiplication (7.1 shoots) was recorded with MS + additives + NAA (0.2 mg/l) + BAP (2.0 mg/l). After subculturing shoot clumps of three or four shoots, early rooting (13.5 days) and 26.67 per cent rooting rate were achieved using ½ MS + additives + NAA (1.0 mg/l). By focusing on axillary shoot proliferation from nodal explants, the research addresses the physiological challenges that have hindered the widespread cultivation of D. stocksii. The findings highlight the challenges in rooting but provide promising results for shoot initiation and multiplication, contributing valuable insights for the large-scale propagation of Dendrocalamus stocksii.
Silviculture and Agroforestry
634.9 / PRI
Development of Efficient in Vitro Regeneration Protocol for Dendrocalamus stocksii (Munro.) Through Nodal Explants - M.Sc. (Forest) - Dharwad University of Agricultural Sciences 2024 - 117 32 Cms
ABSTRACT
Bamboo, a versatile and rapidly growing woody species, holds significant importance across various aspects of life and culture. Among the economically and ecologically potential bamboo species distributed in India, Dendrocalamus stocksii plays an important role in fulfilling those demands. To address gaps, particularly concerning the development of protocols for axillary shoot proliferation using nodal explants, is crucial. Therefore, in vitro propagation study was conducted with the objectives to know the influence of plant growth regulators on in vitro shoot initiation, in vitro shoot multiplication and in vitro rooting of nodal explants.
The research evaluated various nutrient media combinations to optimize shoot initiation, multiplication and rooting processes. The early shoot initiation (3.67 days) was achieved using MS media supplemented with additives+ NAA (0.2 mg/l) + BAP (3.0 mg/l), which also resulted in a maximum of 5 shoots. The greatest shoot length was observed with MS + additives + NAA (0.2 mg/l) + BAP (1.0 mg/l). For in vitro shoot multiplication, the highest sprouting and survival percentages were obtained with MS + additives + NAA (0.2 mg/l) + BAP (4.0 mg/l). The maximum shoot number during multiplication (7.1 shoots) was recorded with MS + additives + NAA (0.2 mg/l) + BAP (2.0 mg/l). After subculturing shoot clumps of three or four shoots, early rooting (13.5 days) and 26.67 per cent rooting rate were achieved using ½ MS + additives + NAA (1.0 mg/l). By focusing on axillary shoot proliferation from nodal explants, the research addresses the physiological challenges that have hindered the widespread cultivation of D. stocksii. The findings highlight the challenges in rooting but provide promising results for shoot initiation and multiplication, contributing valuable insights for the large-scale propagation of Dendrocalamus stocksii.
Silviculture and Agroforestry
634.9 / PRI
