Green Nano Technology Induced Genetic Variation and Diversity Approaches Towards Moisture Stress Tolerance in Triticum Species
Kotabal Sushmita P.
Green Nano Technology Induced Genetic Variation and Diversity Approaches Towards Moisture Stress Tolerance in Triticum Species - M.Sc. (Agri) - Dharwad University of Agricultural Sciences 2024 - 177 32 Cms
ABSTRACT
Wheat species are commercially cultivated in the Indian subcontinent, but experience yield losses due to drought, impacting farmers and the economy. Nanoparticles offer a potential solution to boost crop productivity under drought and climate change. This study investigated genetic variability and diversity in wheat primed with zinc and silicon nanoparticles under moisture-stress and irrigated conditions, focusing on the effects of seed priming on 18 genotypes. Zn and Si nanoparticles were synthesized using eco-friendly green methods using Moringa oleifera and characterized by UV, SEM, XRD and PSA. Under laboratory, among three concentrations and two soaking durations, seed priming with both nanoparticles at 750 ppm for 8 hours significantly enhanced germination, root-shoot length. Biochemical analysis revealed increased dehydrogenase and alpha-amylase activities, likely due to bioactive compounds in the leaf extract. The nanoparticles also improved membrane integrity, indicated by reduced electrical conductivity. Under field conditions, seed priming combined with foliar spray of nanoparticles at 750 ppm improved morpho-physiological traits and moisture stress tolerance in wheat genotypes, along with protein, zinc and iron content. Variability analysis revealed that spike length, grains per spike, peduncle length and productive tillers showed moderate to high GCV, PCV and heritability, indicating additive gene action. Grain yield was positively correlated with biomass, productive tillers and harvest index across all treatments, with a higher direct positive effect, suggesting these traits are key for yield improvement. UAS 446(C) and UASBW 13039 were the best performing, moisture- stress-tolerant genotypes across all treatments according to the stress tolerance index. Genotypes UASBW 12982 and AKDW-2997-16 showed tolerance upon SiNPs treatment, while UAS428(C), a drought-susceptible genotype, exhibited moderate tolerance with both nanoparticles seed priming methods, highlighting their potential to improve moisture stress tolerance. In conclusion, seed priming with Si and Zn nanoparticles significantly improved moisture stress tolerance and nutritional content in wheat respectively.
Genetics and Plant Breeding
581.56 / KOT
Green Nano Technology Induced Genetic Variation and Diversity Approaches Towards Moisture Stress Tolerance in Triticum Species - M.Sc. (Agri) - Dharwad University of Agricultural Sciences 2024 - 177 32 Cms
ABSTRACT
Wheat species are commercially cultivated in the Indian subcontinent, but experience yield losses due to drought, impacting farmers and the economy. Nanoparticles offer a potential solution to boost crop productivity under drought and climate change. This study investigated genetic variability and diversity in wheat primed with zinc and silicon nanoparticles under moisture-stress and irrigated conditions, focusing on the effects of seed priming on 18 genotypes. Zn and Si nanoparticles were synthesized using eco-friendly green methods using Moringa oleifera and characterized by UV, SEM, XRD and PSA. Under laboratory, among three concentrations and two soaking durations, seed priming with both nanoparticles at 750 ppm for 8 hours significantly enhanced germination, root-shoot length. Biochemical analysis revealed increased dehydrogenase and alpha-amylase activities, likely due to bioactive compounds in the leaf extract. The nanoparticles also improved membrane integrity, indicated by reduced electrical conductivity. Under field conditions, seed priming combined with foliar spray of nanoparticles at 750 ppm improved morpho-physiological traits and moisture stress tolerance in wheat genotypes, along with protein, zinc and iron content. Variability analysis revealed that spike length, grains per spike, peduncle length and productive tillers showed moderate to high GCV, PCV and heritability, indicating additive gene action. Grain yield was positively correlated with biomass, productive tillers and harvest index across all treatments, with a higher direct positive effect, suggesting these traits are key for yield improvement. UAS 446(C) and UASBW 13039 were the best performing, moisture- stress-tolerant genotypes across all treatments according to the stress tolerance index. Genotypes UASBW 12982 and AKDW-2997-16 showed tolerance upon SiNPs treatment, while UAS428(C), a drought-susceptible genotype, exhibited moderate tolerance with both nanoparticles seed priming methods, highlighting their potential to improve moisture stress tolerance. In conclusion, seed priming with Si and Zn nanoparticles significantly improved moisture stress tolerance and nutritional content in wheat respectively.
Genetics and Plant Breeding
581.56 / KOT
