Evaluation of Diverse Strains of Pink Bollworm, Pectinophora Gossypiella (Saunders) for Cry Toxin Resistance and its Validation Through Markers (Record no. 70943)

MARC details
000 -LEADER
fixed length control field 02636nam a2200217 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250318162940.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250318b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency UAS Dharwad
041 ## - LANGUAGE CODE
Language code English
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 595.7
Author Label RAK
100 ## - MAIN ENTRY--PERSONAL NAME
Name of Author Rakhesh S
245 ## - TITLE STATEMENT
Title Evaluation of Diverse Strains of Pink Bollworm, Pectinophora Gossypiella (Saunders) for Cry Toxin Resistance and its Validation Through Markers
250 ## - EDITION STATEMENT
Edition Statement P HD
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of Publisher Dharwad
Name of Publisher University of Agricultural Science
Publication Year 2024
300 ## - PHYSICAL DESCRIPTION
Book Pages 200
Book Size 32 Cms
520 ## - SUMMARY, ETC.
Abstract. ABSTRACT<br/> Since the introduction of Bt cotton, its cultivation expanded rapidly from 0.29 lakh ha in 2002-03 to 130.49 lakh ha by 2022-23. By 2015, over 95 % of the Bt cotton area consisted of BG II hybrids with stacked genes (Cry1Ac + Cry2Ab). This widespread adoption exerted significant selection pressure on pink bollworm (PBW), leading to field-scale resistance in India during 2010. Resistance was confirmed through bioassays of PBW populations across India, a process taking 21 days. A survey across 59 locations in India could showed higher boll occupancy of 2.56 in Nagpur and least of 0.71 in Uttara Kannada. Larval parasitoids Bracon lefroyi and Apanteles angaleti have been found actively associated with PBW. Conventional diet incorporation bioassay could show higher resistance in Nagpur strains to Cry1Ac (LC50 of 7.682 µg/ml) and Cry2Ab (LC50 of 12.574 µg/ml). This process typically requires 21-days and the proper Cry toxins hence go tedious and time consuming. For quicker detection of Cry toxin resistance in PBW, Simple Sequence Repeats (SSR) markers have been identified through Bulk Segregation Analysis (BSA) and found that the three SSR markers (notr15F and r15allR2; 164Pgcad5 F and 163Pgcad3 R; gF47 and gR47) were polymorphic between Cry1Ac resistant parents and bulks. Whereas, one SSR marker (gF47 and gR47) was polymorphic between Cry2Ab resistant parents and bulks. Among these specific markers gF47 and gR47 was common to depict Cry1Ac as well as Cry2Ab resistance. Further, the markers were validated in different life stages (larvae, pupae and adults) during 2023-24 with samples from 15 locations having history of reported resistance. Thus, Quick and highly reliable approach for detection of resistance in pink bollworms Indian strains to Cry toxins (Cry1Ac + Cry2Ab together) and /or Cry1Ac has been found through selected SSR markers, which is robust, faster and economical.<br/>
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Subject P h.D (Ento)
700 ## - ADDED ENTRY--PERSONAL NAME
2nd Author, 3rd Author Udikeri S S
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha Item type THESIS
Edition P h.D (Ento)
Classification part 595.7
Call number prefix RAK
Suppress in OPAC No
942 ## - ADDED ENTRY ELEMENTS (KOHA)
-- 595_700000000000000
999 ## -
-- 70943
-- 70943
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Dewey Decimal Classification     University of Agricultural Sciences, Dharwad University of Agricultural Sciences, Dharwad 05/12/2024   595.7/RAK T14117 18/03/2025 1 18/03/2025 THESIS