Transfer of cry 1ac and cry2aa for Pod Borer Resistance Into Wilt Resistant Chickpea (Cicer Arietinum l.) Variety Sa-1 Through Marker Assisted Ackcrossing (Record no. 71095)

MARC details
000 -LEADER
fixed length control field 03649nam a22002057a 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250703110136.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250703b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency University of Agricultural Sciences, Dharwad
041 ## - LANGUAGE CODE
Language code English
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 630.2748
Author Label KAN
100 ## - MAIN ENTRY--PERSONAL NAME
Name of Author Savita Kanthi
245 ## - TITLE STATEMENT
Title Transfer of cry 1ac and cry2aa for Pod Borer Resistance Into Wilt Resistant Chickpea (Cicer Arietinum l.) Variety Sa-1 Through Marker Assisted Ackcrossing
250 ## - EDITION STATEMENT
Edition Statement P hd (Agri)
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of Publisher Dharwad
Name of Publisher University of Agricultural Sciences, Dharwad
Publication Year 2024
300 ## - PHYSICAL DESCRIPTION
Book Pages 115
Book Size 32 Cms
520 ## - SUMMARY, ETC.
Abstract. Chickpea (Cicer arietinum L.) the major pulse crop of both India and World. It is self-pollinated crop. Chickpea is rich source of proteins, fibres and minerals. Pod borer and Fusarium wilt are the major problems affecting chickpea productivity. Hence the crop demands combining of both wilt and pod borer resistance. With the availability of Bt events and wilt resistant variety it was planned to combine both the traits in chickpea using marker assisted breeding. In the present study the cross SA-1 x BS 72C2(cry2Aa) 62,15 and 62 BC2F2, BC1F3 and F4 plants generated respectively, which were PCR positive for cry2Aa and wilt resistance(linked with QTL foc4).From the cross SA-1 x BS 100B (cry1Ac) 2 and 3 BC2F2 and BC1F3 plants generated which were PCR positive for cry2Aa and wilt resistance (linked with QTL foc4).About 27 and 40 BC1F3and F4 plants of the cross SA-1 x BS 72C2(cry2Aa) were raised on sick pot, among these 7 and 16 plant expressed wilt resistance in sick pot and these plants were PCR positive for both cry and wilt resistance. The Cry protein expression studied in the segregating generation of both crosses. After screening through Bt immune blot strips, the Cry protein expression confirmed through qualitative ELISA. Using quantitative ELISA the Cry protein was estimated in segregating populations. The average Cry2Aa protein recorded in segregating generation was 10.18 μg/gm and in transgenic event BS 72C2 it was 10.34 μg/gm. In case of Cry1Ac the mean protein estimated in segregating population was 12.57 μg/gm and in transgenic event 12.44 μg/gm was recorded. Insect bioassays carried out in both plants carrying cry2Aa and cry1Ac gene and it was recorded up to 95 % mortality in both plants. Hence, it was proved that cry2Aa and cry1Ac genes have same effect on pod borer. Recurrent parent genome recovery was studied in BC2F2 and BC1F3 generation plants in both crosses. Only in few plants recurrent genome recovery about 83-90 % was recorded. It may be due to use of less number of SSR markers. Per se performances of segregating populations from both crosses were studied. It was found that the segregating populations were significantly higher than commercial SA-1 variety with respect to height, primary, secondary branches, pods per plant and yield per plant it was observed 40-50 % increased yield in segregating populations over the commercial SA-1 variety. Hence, the segregating population generated from the present study were PCR positive for both pod borer and wilt disease and further cry gene expression was studied through ELISA and insect bioassay. The wilt resistance had been validated through raising on artificial sick pot. It can be concluded that apart from wilt and pod borer resistance the segregating population showed high yield potential. The generated genetic resources with combination of cry1Ac and cry2Aa gene stacking events could be studied.<br/><br/>
700 ## - ADDED ENTRY--PERSONAL NAME
2nd Author, 3rd Author Katageri I S
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha Item type THESIS
Edition P hd (Agri)
Classification part 630.2748
Call number prefix KAN
Suppress in OPAC No
942 ## - ADDED ENTRY ELEMENTS (KOHA)
-- 630_274800000000000
999 ## -
-- 71095
-- 71095
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Dewey Decimal Classification     University of Agricultural Sciences, Dharwad University of Agricultural Sciences, Dharwad 18/06/2025   630.2748/KAN T14158 03/07/2025 1 03/07/2025 THESIS