000 02861nam a2200217 4500
003 OSt
005 20250214111830.0
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040 _cUAS Dharwad
041 _aEnglish
082 _a581.56
_bNAI
100 _aNaikodi Ranjeetaa
245 _aGreen Nano-Technologyinduced Geneticvariation and Diversity Approaches Towards Biofortification in Triticum Species
250 _aM.Sc. (Agri)
260 _aDharwad
_bUniversity of Agricultural Sciences
_c2024
300 _a205
_c32 Cms
520 _aABSTRACT Micronutrient deficiency, specifically zinc (Zn), is prevalent in developing countries. Hence, Biofortification of crops using nanotechnology offers a promising solution to enhance in crops. The present study assessed the effects of green-synthesized zinc nanoparticles (ZnNPs)fromSauropusandrogynusleafextract(ZnNPs-PE)andPseudomonasisolateAUDP 209 (ZnNPs-ME) in wheat genotypes. Thus, synthesized NPs characterized by UV-visible spectroscopy,SEM,XRD,andparticle sizeanalysis. Underlaboratorycondition,amongthree concentration and two soaking durations were, standardized and the seed priming withZnNPs-PE and ZnNPs-ME at 500 ppm for 2 and 4 hours respectively showed improved germination, root length, and shoot length. This enhancement is due to role of ZnNPs in increasing nutrient transporter expression, which boosts nutrient uptake and enzyme activation. Biochemical analyses revealed higher total dehydrogenase and alpha-amylase activities, linked to bioactive compounds in the microbial and leaf extract. Furthur, resulted in lower electrical conductivity, indicating better membrane integrity and reduced ion leakage. ZnNPs seed priming improved protein synthesis, nutrient uptake (Fe and Zn), metabolic efficiency, and minimized protein degradation. ZnNPs seed priming significantly improved yield traits, including grains per spike and grain yield, with high heritability and GAM, making these traits suitable for breeding programs. Path coefficient analysis revealed that ZnNPs seed priming positively affected yield traits through both direct and indirecteffects on grain yield. Among the wheat species, Dicoccum wheat consistently showed the highest levels of zinc, iron and protein contentcompared to bread wheat and durum wheat. Notably, the genotypes DDK 508031, DDK 50931 and UASQ332 showed significant improvement in Zn content, highlighting the potential of ZnNPs applications in enhancing wheat biofortification and nutritional quality. ZnNPs not only significantly enhanced wheat yield but also grain nutritional quality and thus demonstrating their potential for crop biofortification and addressing micronutrient deficiencies.
650 _aGenetics and Plant Breeding
700 _aBiradar Suma S.
942 _cTH
_eM.Sc. (Agri)
_h581.56
_kNAI
_n0
_6581_560000000000000
999 _c70853
_d70853